The basic principle of microbial analysis is to use the characteristics and functions of microorganisms to conduct qualitative and quantitative analysis of compounds in samples. Specifically, the compounds in the sample to be tested are converted into detectable products through microbial bioreduction, biooxidation and other reactions, and then the concentration of the compounds in the sample is calculated by measuring the amount of the products.
The operation procedures of microbial analysis mainly include the following steps:
1. Sample collection: Collect representative samples according to the purpose and requirements of the analysis.
2. Sample pretreatment: Pretreat the collected samples, including crushing, homogenization and other steps, so that the compounds in the sample are fully released.
3. Microbial inoculation and culture: Inoculate the pretreated samples into a specific microbial culture medium, control the appropriate temperature and humidity conditions, and allow the microorganisms to grow and reproduce.
4. Determine the amount of products: During the growth and reproduction of microorganisms, a certain amount of metabolites will be produced. The concentration of the compounds in the sample is determined by measuring the amount of these products. Common determination methods include colorimetry, fluorescence, mass spectrometry, etc.
